SureView Instruments brings its group expertise of over 40 years in India to serve you for your applications that need extraction of intracellular components by electroporation. We manufacture High Voltage Pulsed Electric Field Generators for various applications that include production machines such as for Food processing, or research equipment such as for selective extraction of intracellular components from micro-algae (Nannochloropsis).

Background / Applications:

Electroporation, or electro-permeabilization means forming pores in the cell membrane gently using pulses of electric field for a very short duration. The SureView Pulsed Electric Field Generator is available for this very purpose.

The idea is to puncture the cell so that the intracellular molecules leak out without squashing.
This method helps immensely in food processing, e.g. for making French fries, it helps reduce oil uptake and improves quality. It helps in cold brewing of coffee by extracting without any damage to the ingredients. Food products that are made with PEF have a longer shelf life without much use of preservatives.

Since application of pulsed electric field (PEF) causes a microbial cell lysis, it is of immense importance in renewable fuel research involving microalgae. Utilizing PEF technology results in 90% of the cells being lysed and enhancement in the rate of lipid recovery using ethyl acetate. The four stages in production of fuels from algae are – a. Algae growth, b. Harvesting of Algae, c. Lipid extraction & d. Catalytic conversion of lipids to bio-fuels. PEF is the best way to conduct lipid extraction without the need of long solvent-algae exposure times.

Equipment:

The Pulsed Electric Field Generator comprises of:

• High Voltage pulse transformer
• Programmable Pulse-maker
• Solid state switching circuit
• Control system based on ARM micro-controller

The pulse level, pulse width, number of pulses, interval & cycles are to be fed by the operator in the control system. There is facility to store operation parameters in the memory so that the labor to feed parameters time & again can be avoided.

The HT transformer is provided with proper insulation as well as interlocks to avoid hazards. The specially designed driver circuit takes care of additional safety and avoids any kind of short-circuit.

The electrical design ensures proper safety even at elevated temperatures.

Please email us at mone@svinstruments.co.in for quotation, data-sheet and photographs.